Growth of New Zealand infectious bronchitis virus in cell cultures

Growth of New Zealand infectious bronchitis virus in cell cultures
Peer reviewed


Work between 1972 and 1976 demonstrated that at least four different serotypes of infectious bronchitis (IB) virus were present in New Zealand (Lohr, 1977a). They showed no serologic relationship to a variety of IB virus strains from Australia, the USA and Europe (Lohr, 1976; Lohr and Lecher, 1983). A vaccine (“Poulvac”) was derived from the NZ field strain A in its 49th egg passage (Lohr et ul. 1977) and both field and vaccine strains were shown to be serologically indistinguishable in tracheal organ culture test systems (Lohr and Lecher, 1983; Lecher et al, 1983). In a further attempt to establish distinguishing criteria between the vaccine and field strains of NZ A both were successfully grown in cell cultures. Their growth in chicken kidney (CK) and chick embryo tibroblast (CEF) cultures was investigated and full results will be published elsewhere (Emele et al., 1985). However, we feel that those results relevant to New Zealand should be communicated in this journal. The viruses used were NZ A in the 13th and in the 50th egg passage (“Poulvac”). A number of European field and vaccine strains were also included. The CK cells were from 1-2 week old chickens, the fibroblast cells from 10-day-old SPF embryonated eggs. Digestion was with 0.25Y0 neutral protease (dispase), growth medium was Hank`s MEM without glutamine plus 10% foetal calf serum, 0.5% lactalbumin hydrolysate, antibiotics, and a fungistat. Maintenance medium was growth medium without serum…

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