Evaluation of whole blood collected on to paper discs for the sero-diagnosis of Aujeszky's disease by ELISA

Evaluation of whole blood collected on to paper discs for the sero-diagnosis of Aujeszky's disease by ELISA
Peer reviewed

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) for the quantitative assay of antibody against Aujeszky`s disease virus (ADV) infection is routinely carried out in our laboratory, to certify pigs free of infection with ADV. This test was found to be the most suitable on the grounds of sensitivity, speed and cost, when compared with three other serological tests including the serum neutralisation test (SNT). The use of blood collected onto paper discs (ELISADISC) for ADV serology was recently described. Blood was collected onto peripheral lugs of a filter paper disc (Fig. I) from a nicked ear vein of a pig and air dried. Approximately 10µl of blood was adsorbed onto each lug (six per disc). Each lug was 6mm in diameter. One pig was sampled per disc. On receipt in the laboratory, individual lugs from each disc were snipped off, placed in wells in an ELISA plate and…

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