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A real - time polymerase chain reaction assay for the detection of Mycoplasma agalactiae
Fitzmaurice J, Sewell M, King CM, McDougall S, McDonald WL, O'Keefe JS
New Zealand Veterinary Journal, Volume 56, Issue 5, pp 233-236, Oct 2008
New Zealand Veterinary Journal, Volume 56, Issue 5, pp 233-236, Oct 2008
Article class: Short Communication
Subject Terms: Bacterial, Diagnostic procedures, Disease/defect, Infectious disease, Milk
Animal Type: Goat, Livestock, Production animal, Ruminant, Sheep
Publisher: Taylor and FrancisAbstract
AIM: To develop a real-time PCR for the detection of Mycoplasma agalactiae, using PCR primers targeting the ma-mp81 gene.METHODS: A group of 15 M. agalactiae isolates, 21 other Mycoplasma spp. isolates and 21 other bacterial isolates was used in evaluation of the assay.
RESULTS: All M. agalactiae isolates were detected by the assay and none of the non-target isolates was amplified. The analytical detection limit of the assay was 10 fg of purified genomic DNA and 104 cfu/ml milk inoculated with M. agalactiae. When applied to goat-milk samples collected from three herds free of M. agalactiae infection, the assay had a specificity of 100%.
CONCLUSIONS: The assay would be useful in a diagnostic laboratory, providing specific, sensitive and rapid detection of M. agalactiae.
KEY WORDS: Mycoplasma agalactiae, real-time PCR
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