Survey of bulk tank milk in New Zealand for Mycoplasma bovis , using species - specific nested PCR and culture

Survey of bulk tank milk in New Zealand for Mycoplasma bovis , using species - specific nested PCR and culture
Peer reviewed

Abstract

AIM: To survey the dairy cattle population in New Zealand for the presence or absence of Mycoplasma bovis.
METHODS: A random cross-sectional survey of bulk tank milk from dairy herds in New Zealand based on regionally proportioned sampling, weighted towards herds with a high bulk tank milk somatic cell count (SCC) was used to detect M. bovis at a between-herd prevalence of 2%, with 99% confidence. Bulk tank milk samples collected on-farm were tested using a nested M. bovis PCR, and bacteriological culture employing enrichment in mycoplasma broth and direct plating onto mycoplasma agar.
RESULTS: Mycoplasma bovis was not detected in any of the 244 bulk tank milk samples by either PCR or culture.
CONCLUSIONS: This survey provides further evidence that M. bovis is not present in the dairy cattle population in New Zealand.
KEY WORDS: Mycoplasma bovis, PCR, culture, bulk tank milk, survey

Access to the full text of this article is available:

through another providers website:

If you're a member or subscriber and believe you should have access:

Login

Otherwise:

Register for an account

Request new password

The whole of the literary matter of the New Zealand Veterinary Journal is copyright Taylor and Francis, Downloading this article signifies agreement with the terms and conditions of electronic access.