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A comparison of vitamin B12 levels in the livers and sera of sheep measured by microbiological and radioassay methods

Authors: Millar KR, Penrose ME
Publication: New Zealand Veterinary Journal, Volume 28, Issue 5, pp 97-99, May 1980
Publisher: Taylor and Francis

Animal type: Livestock, Production animal, Ruminant, Sheep
Subject Terms: Clinical pathology, Diagnostic procedures, Cobalt/vitamin B12, Trace elements, Vitamins
Article class: Scientific Article

Abstract: Five radioassay methods (commercial kits) were evaluated for the determination of vitamin B₁₂ in sheep serum. They provided results that correlated significantly (P<0.01) with those obtained using a microbiological assay. One radioassay method was evaluated for analysing vitamin B₁₂ in liver, and the results were in excellent agreement with those obtained by the microbiological method. Little correlation between serum and liver vitamin B₁₂ levels was found. The limited diagnostic value of serum vitamin B₁₂ concentrations for detecting cobalt deficiency in sheep is discussed.

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