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Anti-complementary activity for guinea-pig and sheep complement in serum and Mg2+-EGTA plasma from various animals

Authors: Pulford H, Jonas W, Stankiewicz M
Publication: New Zealand Veterinary Journal, Volume 29, Issue 6, pp 107-108, Jun 1981
Publisher: Taylor and Francis

Animal type: Livestock
Subject Terms: Diagnostic procedures, Immune system/immunology
Article class: Scientific Article

Abstract: Serum and Mg²⁺-ethyleneglycoltetraacetic acid (EGTA) plasma from 14 domestic or laboratory animal species had no anti-complementary activity for sheep complement (C). Serum samples from 8 species and all 14 Mg²⁺-EGTA plasma samples were anti-complementary towards guinea-pig C. Thus, use of sheep C in a warm complement fixation text (with human erythrocytes sensitized with sheep antiserum, IgM, slow or fast γ-globulin antibody), in the presence or absence of Mg²⁺-EGTA, may be a satisfactory method of eliminating anticomplementary activity.

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