Comparison of the enzyme-linked immunosorbent assay (ELISA) and serum neutralisation test for the serodiagnosis of Aujeszky's disease

Authors: Sillars HM, Hobbs IF, Durham PJK
Publication: New Zealand Veterinary Journal, Volume 33, Issue 8, pp 132-135, Aug 1985
Publisher: Taylor and Francis

Animal type: Livestock, Pig, Production animal
Subject Terms: Clinical pathology, Diagnostic procedures, Viral
Article class: Scientific Article

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Abstract: The serum neutralisation test (SNT) and the indirect enzyme-linked immunosorbent assay (ELISA) for Aujeszky`s disease were compared, utilising 3202 sera from Aujeszky`s disease free pig herds, and 304 SNT reactor and 245 non-reactor sera from Aujeszky`s disease infected piggeries. ELISA was found to give good discrimination between positive and negative sera, results showing 96.9% and 99.7% agreement with the SNT in classifying positive and negative reactor pigs respectively. The ELISA appeared to detect a slightly higher proportion of reactors than did the SNT. Absorbance values obtained with ELISA showed a high degree of overall correlation with SW titres (r = 0.916), though correlations were lower when applied to individual sera. The ELISA was considered to be a rapid and convenient procedure, offering many advantages over the SNT for routine use.

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