The prevalence of Shiga toxin-producing Escherichia coli in cattle and sheep in the lower North Island, New Zealand

Authors: Cookson AL, Taylor SCS, Attwood GT
Publication: New Zealand Veterinary Journal, Volume 54, Issue 1, pp 28-33, Feb 2006
Publisher: Taylor and Francis

Animal type: Cattle, Human, Livestock, Production animal, Ruminant, Sheep
Subject Terms: Abdomen, Alimentary system/gastroenterology, Bacterial, Infectious disease, Disease/defect, Disease transmission, Epidemiology, Diet/rations/food, Public health, Zoonosis
Article class: Scientific Article

---

Abstract: AIM: To genotype Escherichia coli cultured from the faeces of healthy cattle and sheep in the lower North Island, in order to investigate the possible role of ruminants as a reservoir for Shiga toxin-producing E. coli (STEC) in New Zealand.
METHODS: A total of 952 strains of E. coli were isolated on selective media, from faecal swabs from 319 animals (187 cattle and 132 sheep) from four sites in the Manawatu and Rangitikei regions of New Zealand. A multiplex polymerase chain reaction (PCR) was used to genotype the E. coli isolates, using amplification of Shiga toxin genes (stx1 and stx2) and the E. coli attaching and effacing gene (eae).
RESULTS: Isolates of E. coli were cultured from swabs from 178/187 (95.2%) cattle and all 132 (100%) sheep. Ninety-nine (10.4%) of the isolates were stx1 only, 83 (8.7%) stx2 only, 33 (3.5%) stx1 and stx2, 23 (2.4%) stx1 and eae, one (0.1%) stx2 and eae, and 115 (12.1%) were eae only. Overall, 51 (27.3%) cattle and 87 (65.9%) sheep were stx-positive, whereas 69 (36.9%) cattle and 36 (27.3%) sheep were eae-positive.
CONCLUSIONS: Both healthy cattle and sheep are asymptomatic reservoirs of STEC in New Zealand. Direct contact with cattle and sheep or consumption of water or foodstuffs contaminated with cattle of sheep faeces may represent a significant source of infection for humans.
KEY WORDS: Shiga toxin-producing Escherichia coli, STEC, stx, eae, cattle, sheep, ruminant, food safety, public health

---